Comparative Transcriptome Analysis of Caryophyllene- Treated <i>Helicobacter pylori</i>

Helicobacter pylori (H. pylori) establishes long-term infections associated with severe gastric diseases such as peptic ulceration and cancer. Exposure to an antibacterial agent can help regulate the expression levels of its pathogenic genes. In this study, we analyzed transcriptional changes in H. genes induced by β-caryophyllene. We used next-generation sequencing (NGS) analyze RNA changes, reverse transcription-polymerase chain reaction (RT-PCR) was performed required verify results. The NGS results showed that 30 out 1,632 were expressed differentially β-caryophyllene treatment. Eleven DNA replication, virulence factors, T4SS components significantly downregulated. RT-PCR confirmed treatment reduced 11 (dnaE, dnaN, holB, gyrA, cagA, vacA, secA, flgE, virB2, virB4, virB8) following These suggest modulate various determinants be a potential therapeutic for infection.